Tweaking the Spliceosome Rubik’s Cube by Prp28 RNA Helicase
Tweaking the Spliceosome Rubik’s Cube by Prp28 RNA Helicase ——
拍攝日期：2021/09/27主講人：張典顯 研究員 (中研院基因體研究中心)主持人：陳沛隆 所長 (臺大基因體暨蛋白體醫學研究所)Splicing, a key step in the eukaryotic gene-expression pathway, converts precursor messenger RNA (pre-mRNA) into mRNA by excising introns and ligating exons. This task is accomplished by the spliceosome, a macromolecular machine that must undergo sequential conformational changes to establish its active site. Each of these major changes requires a dedicated DExD/H-box ATPase, but how these enzymes are activated remain obscure. Here we show that Prp28, a yeast DEAD-box ATPase, transiently interacts with the conserved 5’ splice-site (5’SS) GU dinucleotide and makes splicing-dependent contacts with the U1 snRNP protein U1C, and U4/U6.U5 tri-snRNP proteins, Prp8, Brr2, and Snu114. We further show that Prp28’s ATPase activity is potentiated by the phosphorylated Npl3, but not the unphosphorylated Npl3, thus suggesting a strategy for regulating DExD/H-box ATPases. We propose that Npl3 is a functional counterpart of the metazoan-specific Prp28 N-terminal region, which can be phosphorylated and serves as an anchor to human spliceosome.https://www.genomics.sinica.edu.tw/index.php/tw/news/lastest-news/662-prp28 (Hanzi version)https://www.genomics.sinica.edu.tw/index.php/en/news/latest-news?z2vub=298284 (English version)►►臺大演講網Website: http://speech.ntu.edu.twFacebook: http://www.facebook.com/ntuspeech
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